Avian Metapneumovirus (aMPV) Real-Time PCR Subgroups A, B and C
1) Tracheal or oropharyngeal swab that includes sampling the choanal cleft, 2) infraorbital sinus exudate or fluid; pooled up to 5 swabs per tube. Use Synthetic swab with a plastic shaft, 3)Drinker Swabs (pooled up to 5/bag); use 3X3 gauze swabs.
Swabs may be pooled up to 5 per tube. Consider requesting both PCR and Virus Isolation for this disease. For the best chance of detecting or isolating this virus, please submit fresh samples as soon as clinical signs are noted. The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. PCR Testing is done at the Minnesota Poultry Testing Laboratory. If requested, virus isolation is done at the MVDL. You may send samples to either lab and they will be forwarded as needed. For faster turnaround time you may also collect duplicate samples and send the PCR samples to MPTL, and virus isolation samples to MVDL.
Test update
Avian Metapneumovirus (aMPV) Real-Time PCR Subgroups A, B and C: Starting on July 1, 2025, we are implementing a new testing schedule for aMPV PCR (subgroups A, B, and C). Please note the following changes:
Testing will now be performed on Wednesdays and Fridays only.
Turnaround time is 5–7 business days, as we must account for courier-delivered samples that may arrive later in the week.
Samples received by end of day Tuesday will be tested on Wednesday.
Samples received by noon on Thursday will be tested on Friday.
If you have urgent testing needs outside of this schedule, please contact the MPTL Laboratory Manager to discuss whether off-cycle testing can be arranged.