Whole Genome Sequencing from clinical samples or <15 viral isolates
Specimen Requirements: 1) At least 400 uL (0.4 mL) 2) at least 2 g tissue
We use Illumina technology and aim for at least 1 million reads per sample. However, we cannot guarantee the success of NGS procedures as this technique is the unbiased amplification of all nucleic acids in the sample. The number of reads for a pathogen of interest may not be sufficient to produce a full genome. In those instances, a draft genome may be produced and additional sequencing may be necessary to fill in gaps in the genome. Additional sequencing options and costs will be discussed if needed. When sufficient reads are available, full or draft genomes will be reported with annotations, comparison with vaccine or reference sequences, recombinant/reassortment analysis, and dendrograms.