Notice of location change for Avian Necropsy & Pathology services
All Avian Necropsy & Pathology services are now being provided by the MVDL, St. Paul, until further notice. All NPIP testing, diagnostic avian serology and avian PCR testing will continue to be tested at MPTL in Willmar. Please submit, or ship, your samples directly to the lab that will service the samples.
On January 3rd, 2023 there will be a change to the display formatting of Summary results on the University of Minnesota Veterinary Diagnostic Laboratory Results Access web portal and in nightly Summary emails
The Minnesota Veterinary Diagnostic Laboratory is returning the Actinobacillus suis (A. suis) PCR as a test option for a variety of sample types. An improved real time PCR test was developed for the detection of A. suis from porcine oral fluids, lung, joint, joint swabs, pooled tissues, pleura, pericardium, peritoneum, tonsil scrapings, biopsies, nasal swabs, oropharyngeal swabs, tonsil swabs, bone swabs, serum, bacterial isolates, fetal tissues, and subcutaneous skin swabs (ear/other skin lesions). The test will be run weekly on Monday at a cost of $27.00. As with most tests, samples should be at the lab by noon the day before the test is scheduled to run ( by noon Friday for the A.suis PCR). Off schedule/emergency testing may be possible after consultation with a food animal diagnostician.
The A. suis primers and probe target the 23S ribosomal gene and are adapted from the reference article Kariyawasam, Subhashinie, et al. “Development of a Real-Time Polymerase Chain Reaction Assay for Detection of Actinobacillus Suis in Porcine Lung.” Updates were made to modernize the enzyme kit used in the assay as well as the probe prior to the start of validation. The sequence of the primers and probe remained the same as the publication. The A. suis PCR test has a detection limit of 50 copies/reaction. The assay also has an exogenous internal control added during extraction.
Effective May 1, 2023, the Minnesota Veterinary Diagnostic Laboratory will be changing the reflex testing protocols for influenza A virus testing in swine samples.
For the past many years we've performed reflex subtyping (H1/H3/N1/N2 PCRs) and set up virus isolation (VI) on the sample with the lowest Ct value for the case, as long as the detection Ct value is <30.
We will now be changing our protocols to mirror the USDA Swine Influenza A Virus Surveillance Program, and will only be performing reflex subtyping and virus isolation on oral fluid samples with detection Ct values of <20, and on nasal swab/wipe samples and lung samples with detection Ct values of <25, unless specifically instructed otherwise.
If you would like your samples to have reflex subtyping and/or virus isolation performed even if they fall outside of the the above detection Ct criteria, please indicate this preference on your submission forms, or you may request these additional tests via your Results Access webportal or by calling or emailing the laboratory once the detection results are reported.
Please feel free to contact Dr. Matt Sturos (email to [email protected] or call 612-625-0947) with any questions or concerns you may have with this upcoming change.
2) A new fee which will be applied only to cases for which we receive samples that are not in the preferred sample containers, and/or in which samples containers are received with animal IDs written on the containers (e.g. - 14207, 34591, Daisy, Mabel, ...) instead of being serially numbered (e.g. 1, 2, 3, 4,...). Individual animal IDs should be recorded on the submission form, on a separate piece of paper, or in a spreadsheet file provided to the lab for direct upload into our information management system, with the animal IDs arranged in the correct order so as to correspond to the serial numbers you apply to the sample containers (e.g. - 1 = 14207, 2 = 34591, 3 = Daisy, 4 = Mabel,...).
This new BVDV pooled ear notch organization fee will be applied on a per-pool basis, at $20 per pool tested, for cases meeting the criteria stated above. Cases received with samples in the preferred containers and for which the containers are serially numbered will NOT incur this additional per-pool fee. Cases received after January 1, 2023, will be evaluated for the applicability of this fee.
3) The third item clarifies a policy we will be applying to any PCR positive or PCR suspect pools. For any pool with a positive or suspect PCR result, we will be automatically testing the individual samples which make up the pool using the BVD antigen capture ELISA (ACE) test. Per our website, each BVDV ACE test is $5.46 per sample tested (Link to BVDV ACE test online catalog entry). This will allow for the individual identification of BVDV infected animals.